#Unstained protein ladder western blot plus
#Unstained protein ladder western blot series

The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. BenchMark Protein Ladder consists of 15 unstained recombinant proteins ranging in molecular weight from 10 to 220 kDa. The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. Stronger blot persistence provides enhanced: Confidence after western blot processing. Increased brightness makes it easier to: Identify target proteins on the gel or blot. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD) Improved Dual Color Standards are brighter than the competition on both gels and blots.Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD).

#Unstained protein ladder western blot plus

Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots.

Accurate protein sizing on SDS-polyacrylamide gels and Western blots.Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

The methanol concentration in a western blot should be between 10 20.

Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25☌), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol Choosing a protein ladder for your application Type Marker Cat. however, can be a problem as the proteins can become fixed in the gel.

The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. Locating a protein of interest for excision from an unstained preparative gel. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. Mark12 Unstained Standard consists of 12 polypeptides in the range of 2.5200 kDa. The 150kDa band is of greater intensity and serves as a reference band. The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver.

Quality tested-each lot evaluated by SDS-PAGE and Western blotting.

Browse Bio-Rads wide range of quality prestained and unstained protein standards.

Tagged-each protein in the ladder contains an integral Strep-tag™ II Sequence Details on blotting technology, available products, and tips and techniques.

Storage Buffer Approximately 0.10.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5. Precision Plus Protein All Blue Standards are a mixture of ten blue-stained recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). Sizing of proteins on SDS-PAGE and Western blots.

Reference band-150kDa band has greater intensity for easy orientation Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

Sharp bands-excellent accuracy for detection in with coomassie or silver stains.

Ready-to-use-supplied in a loading buffer for direct loading on gels.

Size range-8 proteins spanning 60 to 250kDa.

#Unstained protein ladder western blot series

Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.įeatures of PageRuler Unstained High Range Protein Ladder: Prestained Protein Marker, Broad Range (11-190 kDa) is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands.